Mouse phenylalanine hydroxylase. Homology and divergence from human phenylalanine hydroxylase.
نویسندگان
چکیده
The laboratory mouse represents an important model for the study of phenylalanine metabolism and the pathochemistry of phenylketonuria, yet mouse phenylalanine hydroxylase (PAH) has not been extensively studied. We report the cloning and sequencing of a mouse PAH cDNA, the expression of enzymic activity from the mouse PAH cDNA clone and the identification of mouse PAH and human PAH by two-dimensional PAGE of liver samples. These data confirm the expected homology of mouse PAH and human PAH and suggest differences in the primary sequence and the phosphorylation state of the two enzymes.
منابع مشابه
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 267 2 شماره
صفحات -
تاریخ انتشار 1990